BBA Gene Regulatory Mechanisms includes reports that describe novel insights into mechanisms of transcriptional, post-transcriptional and translational gene regulation. Special emphasis is placed on papers that identify epigenetic mechanisms of gene regulation, including chromatin, modification, and remodeling. This section also encompasses mechanistic studies of regulatory proteins and protein complexes; regulatory or mechanistic aspects of RNA processing; regulation of expression by small RNAs; genomic analysis of gene expression patterns; and modeling of gene regulatory pathways. Papers describing gene promoters, enhancers, silencers or other regulatory DNA regions must incorporate significant functions studies.The journal does not favorably review manuscripts identifying a miRNA-target pair without additional insights into the repression mechanism or significant advances in understanding regulatory pathways. In addition, the following elements should be an integral part of the study:?In silico prediction of miRNA targets must be experimentally verified using appropriate luciferase constructs and assays;?To exclude non-functional miRNA/mRNA interactions a reporter system including the whole 3'UTR of the target gene downstream the "luciferase" or GFP should be considered;?Any miRNA modulation should be validated by measuring the expression of the putative protein

BBA基因調(diào)控機(jī)制包括對轉(zhuǎn)錄、轉(zhuǎn)錄后和翻譯基因調(diào)控機(jī)制的新見解的報告。特別強(qiáng)調(diào)的論文，以確定表觀遺傳機(jī)制的基因調(diào)控，包括染色質(zhì)，修改和重塑。本節(jié)還包括調(diào)控蛋白和蛋白復(fù)合物的機(jī)制研究;RNA處理的調(diào)控或機(jī)制方面;小rna對表達(dá)的調(diào)控基因表達(dá)模式的基因組分析;以及基因調(diào)控通路的建模。描述基因啟動子、增強(qiáng)子、沉默子或其他調(diào)節(jié)DNA區(qū)域的論文必須包含重要的功能研究。如果沒有對抑制機(jī)制的深入研究或在理解調(diào)控途徑方面的重大進(jìn)展，該雜志不會對鑒定mirna -靶點(diǎn)對的手稿進(jìn)行正面評價。此外，下列要素應(yīng)成為研究的一個組成部分:?在miRNA目標(biāo)的硅預(yù)測中，必須使用合適的熒光素酶構(gòu)建物和檢測方法進(jìn)行實驗驗證;?為了排除非功能性miRNA/mRNA相互作用，應(yīng)該考慮包括“熒光素酶”或GFP下游目標(biāo)基因的整個3’utr的報告系統(tǒng);?任何miRNA的調(diào)節(jié)都應(yīng)該通過測量假定蛋白的表達(dá)來驗證